Variation in phosphotransferase (PTS) enzyme II systems in O. oeni . A. The occurrence and location of PTS enzyme II clusters across fourteen strains of O. oeni. The presence of a particular ORF in a specific strain is indicated (blue shading). Pseudogenes are also shown (grey shading). Individual PTS clusters are grouped by solid lines. B. A novel PTS IIC-glycosidase gene fusion in O. oeni strains AWRIB422 and AWRIB548. The two alternative genotypic arrangements are shown with areas of near complete sequence homology indicated by green shading. Genomic loci that display functional similarity (e.g. both proteins are PTS IIA subunits) are also indicated by pink shading.