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Table 1 Box C/D snoRNA genes in E. histolytica

From: Computational prediction and validation of C/D, H/ACA and Eh_U3 snoRNAs of Entamoeba histolytica

snoRNA genes Len. Seq. Modification Antisense element Scaffold Start End Location
(nt) (%)
**Me-Eh-SSU-G1296 78 92% SSU-G1296 12nt(5') 100% DS571223 24176 24254 IR
    SSU-G1298 10nt(5’) 100%     
    SSU-G1195 10nt(5’) 100%     
Me-Eh-SSU-U1024 80 96% SSU-U1024 14nt(5') 95% DS571261 44605 44684 IR
    SSU-U1822 11nt(5’) 98%     
**Me-Eh-SSU-A83 78 100% SSU-A83 16nt(5') 100% DS571196 58225 58327 IR
    SSU-U87 12nt(5’) 100%     
Me-Eh-SSU-G41 68 93% SSU-G41 11nt(5’) 100% DS571147 177417 177350 IR
Me-Eh-SSU-A431 68 94% SSU-A431 13nt(5') 100% DS571331 10236 10303 IR
Me-Eh-SSU-U871 80 96% SSU-U871 20nt(5’) 95% DS571673 2402 2481 NA
*Me-Eh-SSU-G1535 82 93% SSU-G1535 12nt(5') 100% DS571215 31121 31040 IR
    LSU-G2053 9nt(5') 100%     
Me-Eh-SSU-A27 66 100% SSU-A27 11nt(5') 100% DS571226 26372 26307 IR
Me-Eh-SSU-A1830 83 88% SSU-A1830 11nt(5') 100% DS571152 29351 29433 EHI_049420 (+)
Me-Eh-SSU-A836 103 -- SSU-A836 13nt(5’) DS571152 99242 99140 IR
Me-Eh-SSU-G1152 60 91% SSU-G1152 12nt(3') 100% DS571335 19522 19581 IR
Me-Eh-SSU-G628 97 -- SSU-G628 10nt(5’) DS571451 15436 15532 IR
      DS571177 52928 52831  
Me-Eh-SSU-A1183 82 -- SSU-A1183 10nt(5’) DS571164 22795 22876 IR
    SSU-G1836 13nt(5’)     
    SSU-A1485 9nt(5’)     
    LSU-A520 12nt(3’)     
    LSU-U1210 12nt(5’)     
    LSU-A145 10nt(3’)     
Me-Eh-SSU-A790 68 94% SSU-A790 10nt(5’) 100% DS571171 51701 51634 IR
    SSU-A1496 11nt(5’) 100%     
    LSU-A801 11nt(5’) 100%     
    LSU-A1834 10nt(5’) 100%     
    LSU-A2555 11nt(5’) 100%     
Me-Eh-SSU-C1805 63 96% SSU-C1805 10nt(5') 100% DS571145 496851 496789 IR
Me-Eh-LSU-A928a 69 97% LSU-A928 11nt(5') 100% DS571323 13072 13140 IR
Me-Eh-LSU-A928b 66 98% LSU-A782 11nt(5') 100% DS571163 50734 50669 IR
    LSU-A928 9nt(5’) 100%     
    LSU-A1034 9nt(5’) 100%     
Me-Eh-LSU-U1868 101 92% LSU-U1868 13nt(5’) 92.3% DS571175 28933 28833 IR
Me-Eh-LSU-U3580a 103 -- LSU-U3580 19nt(5’) DS571304 677 575 IR
Me-Eh-LSU-U3580b 105 -- LSU-U3580 19nt(5’) DS571305 36390 36494 IR
Me-Eh-LSU-A785 62 96% LSU-A785 13nt(5') 100% DS571416 15678 15739 IR
Me-Eh-LSU-G2958 70 97% LSU-G2958 13nt(5') 100% DS571205 22350 22419 IR
*Me-Eh-LSU-A3089 71 92% LSU-A3089 11nt(5’) 100% DS571180 41005 40935 IR
*Me-Eh-LSU-C2414 69 97% LSU-C2414 11nt(5') 100% DS571473 957 1025 IR
Me-Eh-LSU-G926 59 98% LSU-G926 13nt(3') 100% DS571150 13447 13389 IR
Me-Eh-LSU-U1018 69 -- LSU-U1018 11nt(5’) DS571215 62034 62102 IR
    LSU-U2783 14nt(5’) DS571316 3067 2999  
Me-Eh-LSU-G1028 61 87% LSU-G1028 14nt(3’) 100% DS571174 92482 92422 IR
Me-Eh-LSU-U1176a 109 94% LSU-U1176 14nt(5') 100% DS571307 17712 17820 IR ▲
Me-Eh-LSU-U1176b 109 94% LSU-U1176 14nt(5') 100% DS571419 10643 10535 IR
Me-Eh-LSU-U1176c 109 93% LSU-U1176 14nt(5') 100% DS571792 3710 3820 IR
Me-Eh-LSU-A2333 128 93% LSU-A2333 12nt(3') 100% DS571208 15564 15691 IR
**Me-Eh-LSU-A228 72 97% LSU-A228 13nt(5') 100% DS571397 17920 17991 EHI_003940
Intron of gene
40 S ribosomal protein S4, putative
**Me-Eh-5.8 S-U84 62 86% 5.8 S-U84 18nt(3’) 91% DS571194 27534 27595 3’UTR
*Me-Eh-5.8 S-A92 115 85% 5.8 S-A92 11nt(5’) 94% DS571180 76405 76291 EHI_118830 (−) ■
  1. ** snoRNAs validated by RT-PCR and Northern, * validated only by RT-PCR.
  2. Note: “Len.” denotes length of the snoRNA genes, “Seq.” is sequence identity of corresponding snoRNA genes in E. dispar, “Antisense element” denotes length of antisense element in E. histolytica and its sequence identity with E. dispar. “IR”, intergenic region, “NA”, no annotation. snoRNA located close to ribosomal protein genes , downstream to rRNA methyltransferase gene ▲, close to C/D box snoRNP (fibrillarin) ■. (+) and (−) represents snoRNA in sense and antisense orientation with respect to host gene.