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Table 3 Orphan snoRNA genes (C/D and H/ACA) in E. histolytica

From: Computational prediction and validation of C/D, H/ACA and Eh_U3 snoRNAs of Entamoeba histolytica

snoRNA genes Len Seq Modification Antisense element Scaffold Start End Homology Yeast Human Location
(nt) (%)
EhCDOrph1 95 95% unknown unknown DS571162 42554 42648 unknown EHI_155390 (+)
EhCDOrph2 87 94% unknown unknown DS571301 21222 21308 unknown IR
EhCDOrph3 107 94% unknown unknown DS571358 4592 4698 unknown IR
EhCDOrph4 91 96% unknown unknown DS571422 5594 5684 unknown IR
EhCDOrph5 84 94% unknown unknown DS571468 9619 9702 unknown IR
EhCDOrph6 94 -- unknown unknown DS571178 12358 12451 unknown 3'UTR/IR
EhCDOrph7 94 -- unknown unknown DS571178 13726 13819 unknown 3'UTR/IR
EhACAOrph1 115 91% unknown unknown DS571172 5407 5293 unknown IR
EhACAOrph2 135 93% unknown unknown DS571155 108854 108988 unknown IR/5’UTR
**EhACAOrph3 137 94% unknown unknown DS571258 10028 9892 unknown IR
EhACAOrph4 122 90% unknown unknown DS571205 43143 43022 unknown IR
EhACAOrph5 129 - unknown unknown DS571332 15845 15717 unknown IR
**EhACAOrph6 158 - unknown unknown DS571298 19208 19365 unknown IR
EhACAOrph7 130 - unknown unknown DS571219 6608 6737 unknown IR
EhACAOrph8 131 88% unknown unknown DS571162 44597 44467 unknown IR
EhACAOrph9 120 89% unknown unknown DS571164 102500 102619 unknown IR
EhACAOrph10 149 87% unknown unknown DS571179 6844 6696 unknown EHI_093690 (−)
EhACAOrph11 139 94% unknown unknown DS571299 12352 12214 unknown EHI_099700 (−)
EhACAOrph12 134 91% unknown unknown DS571402 6404 6271 unknown EHI_067510 (−)
**EhACAOrph13 137 95% unknown unknown DS571501 3747 3883 unknown EHI_171990 (+)
**EhACAOrph14 153 97% unknown unknown DS571295 13935 14087 unknown EHI_082520 (−)
*EhACAOrph15 148 91% unknown unknown DS571166 95075 95222 unknown EHI_127390 (−)
  1. ** snoRNAs validated by RT-PCR and Northern, * validated only by RT-PCR.
  2. Note: “Len.” denotes length of the snoRNA genes, “Seq.” is sequence identity of corresponding snoRNA genes in E. dispar, “IR”, intergenic region, “NA”, no annotation. snoRNA located close to ribosomal protein genes . (+) and (−) represents snoRNA in sense and antisense orientation with respect to host gene.