Abnormal expression of Nek8 along the cilia in the LPK. Immunohistochemical triple labeling for acetylated α-tubulin, γ-tubulin and Nek8 in LPK animals. The use of both γ-tubulin (green, as a marker for the basal body) and acetylated α-tubulin (green, as a marker for the cilia) confirmed the altered expression of Nek8 (red) along the length of the cilia in the LPK. Two examples illustrating multiple cilia are presented in panels A and B. Round circle indicates γ-tubulin while the dashed line highlights the cilia as identified using acetylated α-tubulin. Scale bars in each panel represent 5μm.