Confirmation of RNA seq results by semi-quantitative RT-PCR. Final point semi-quantitative RT-PCR was used to validate some of the genes found to be differentially expressed by RNA seq. CDR1 was used as a positive control of a gene induced in the Gu5 strain. ACT1 was used as a reference gene equally expressed in Gu5 and Gu4. The genes for which the PCR results did not confirm the RNA seq results are marked by a cross on the right of the gel picture.