Expression of genes surrounding speG . (A) RT-PCR analysis of the expression of the eight or nine genes surrounding speG in GGS_124 (SDSE) and MGAS6180 (GAS). The amino acid identity of each gene (%) in these two strains is indicated below each gene. (B) Putative predicted promoter regions at -10 bp and -35 bp of speG in GGS_124 and MGAS6180. The mutation in the putative -10 bp region in GGS_124 made it no longer a putative promoter region; it is therefore enclosed in a dotted line box.