Real time RT/PCR of whole organ cassini expression in control and LPS injected mice. (A, B) Cassini expression in different murine tissues and 5 hrs after I.P. injection with 15 mg/kg E. coli LPS. Expression was verified twice in sets of control and LPS-treated mice (n = 2). Triplicate real-time values are represented as percent of gapdh. *p < 0.05 LPS spleen compared to control spleen. ***p < 0.001 LPS thymus compared to control thymus. (C) Real time RT/PCR on RNA from control and LPS (1 μg/mL) treated splenocytes isolated from a control mouse (n = 1).