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Table 1 Statistics of sRNA sequences from B. napus

From: Identification of miRNAs and their targets from Brassica napus by high-throughput sequencing and degradome analysis

  Redundant Non-redundant
  Number of counts % of total Number of unique sequence % of total
Raw reads 13020106 \ 2149116 \
Adaptor removed 30673 0.24 25713 1.2
Junk Filter a 5182 0.04 2362 0.11
Length filter 1379794 10.6 646357 30.08
Simple sequence filter b 69175 0.53 11859 0.55
Copy number <3 1219472 9.37 1130389 52.6
Hit mRNA, RFam, Repbase 9877816 75.87 57637 2.68
Mappable 437994 3.36 30400 1.41
  1. a the sequences are filtered out if they contain 3 Ns (N is undetermined nucleotide) and only A and C without G and T, vice versa; b the simple sequences are filtered out if they contain 2 Ns (N is undetermined nucleotide), 7 consecutive As, 8 consecutive Cs, 6 consecutive Gs or 7 consecutive Ts and 10 repeats of any dimer, 6 repeats of any trimer, or 5 repeats of any tetramer. These numbers were from the statistics of miRbase ver16.