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Table 3 Comparison of the current methods versus PBS.

From: Pair-barcode high-throughput sequencing for large-scale multiplexed sample analysis

Samples number PSS 1 SBS 2 Combination of PSS 1 and SBS 2 PBS
10 Allowing up to 8 samples 10 barcoded oligos 4 segments with 3 barcoded oligos 7 barcoded oligos
(4 F-barcodes and 3 R-barcodes)
100 N/A 100 barcoded oligos 8 segments with 13 barcoded oligos 20 barcoded oligos
(10 F-barcodes and 10 R-barcodes)
1000 N/A 1000 barcoded oligos 8 segments with 125 barcoded oligos 67 barcoded oligos
(34 F-barcodes and 33 R-barcodes)
10000 N/A 10000 barcoded oligos 8 segments with 1250 barcoded oligos 200 barcoded oligos
(100 F-barcodes and 100 R-barcodes)
  1. 1 PSS, Physical Space segregation
  2. 2 SBS, Single barcode sequencing