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Table 2 Primers used for real-time RT-PCR

From: Serpins in rice: protein sequence analysis, phylogeny and gene expression during development

Primer Sequence Amplicon size (bp)
OsSRP-QKG fwd 5-ACATGCGGAAGCTGGGCGTGA-3 167
 rvs 5-TTGCATCATAACCACGGCGGTG-3  
OsSRP-LGC fwd 5-ACAAGACGAACGCGGCGGAGAC-3 133
rvs 5-TGGTAGACGGCCGACACGACGA-3  
OsSRP-LRS fwd 5-ACGCGGCAAGTTACTGTCGGGC-3 228
 rvs 5-TGCAGCAGCAGCCTCAGTCCC-3  
OsSRP-FRS fwd 5-GGCTGCGAAGCTGAACTCTGAAC-3 197
 rvs 5-TCTCTGGAGAACCCACCATCCCA-3  
OsSRP-PTY fwd 5-AGCGAGCAGGAAGTCTCCCCG-3 155
 rvs 5-GGCGGCGTGTTCACACTCACA-3  
OsSRP-PLP fwd 5-CCTCCGGGAAGCTGAATTCTCTG-3 220
rvs 5-TACTGTTCCAGAGACCTCCTCCC-3  
OsSRP-FAS fwd 5-TCAAGCCGTTCGTGGCGGACC-3 170
 rvs 5-CGCCGCCGATGCGAAGGTAAG-3  
OsSRP-FLC fwd 5-AAGATGGCAGTGGCGTCGTCCG-3 303
 rvs 5-CTTCATCGTGCAGGCCGTGG-3  
  1. BLASTN searches against the rice genome using the primers as query sequences were performed to check for the possibility of non-specific hybridisation. fwd = forward; rvs = reverse.fwd = forward; rvs = reverse.