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Table 1 EGFP expression patterns of mutant Enhancer-trap appb BACs

From: Using BAC transgenesis in zebrafish to identify regulatory sequences of the amyloid precursor protein gene in humans

Enhancer -trap BAC injected

Intron enhancers

Average of 4 or 6 injections

 

Deletions from CT-repeat end

# of eggs injected

# of embryos survived

# of expression in neurons

Δ94C

CT+ve

75

32

10

Δ2C, Δ76C, Δ51D

CT-ve

90

40

8

Δ5C, Δ14C, Δ17D, Δ21D

CT-ve, SOX 5-ve

110

33

9

Δ17C, Δ9D, Δ14D

CT-ve, SOX 5-ve, E4BP4-ve

140

70

0

 

Deletions from GATA3 end

   

Δ46C, Δ27D, Δ28D

GATA3-ve

105

42

12

Δ3C, Δ4C, Δ1D

GATA3-ve, OCT1-ve

150

100

8

Δ28C, Δ13D, Δ48D, Δ52D

GATA3-ve, OCT1-ve, XFD1-ve

130

55

0

  1. Mutations were engineered into the intron 1 enhancer (IE) of the enhancer-trap and inserted into appb BACs C and D. Structures of mutant enhancer-trap BACs shown schematically in Panel B of Figure 2, and their expression patterns summarized here in Table 1.
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BMC Genomics

ISSN: 1471-2164

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