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Table 2 The detailed results of our testing on the Phalaenopsis ESTs data with different parameters

From: An efficient RNA interference screening strategy for gene functional analysis

Parameters d T  = 3,d N  = 5,L = 21 d T  = 4,d N  = 5,L = 21 d T  = 4,d N  = 6,L = 21
Number of enumerated subsequences 191,087 191,087 191,087
Number of subsequences (after Far_neighbor and powerful subsequence examination) 173,106 188,889 117,203
Number of qualified siRNAs 100,890 106,188 6,443
Number of non-redundant qualified siRNAs 487 2,147 352
Average number of target genes of a qualified siRNA 1.0583 1.1163 1.0852
Number of qualified siRNAs (number of target gene > 1) 5,565 10,863 491
Average number of target genes of qualified siRNAs (number of target gene > 1) 2.0577 2.1370 2.1181
Number of selected siRNAs in first round analysis 142 94 162
Number of candidate-genes examined in first round analysis 229 229 227
Average examination frequency of each candidate-gene 1.153 1.170 1.110
  1. From these result we can see that the number of qualified siRNA has important influence on the number of used siRNAs in first round analysis.