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Table 3 Off-target analysis of HFA and SRSF hit lists

From: Advances in genome-wide RNAi cellular screens: a case study using the Drosophila JAK/STAT pathway

On target Predicted off target  
On target - dsRNA On target - Gene On target - score Off target – Flybase ID Off target - Gene Off target - dsRNA Off target - score  
HFA16984 larp -3.1 FBgn0003013 osa HFA17022 -4.2 *
HFA18710 RpS14a -6.1 FBgn0004404 RpS14b HFA18711 -4.9 *
HFA19901 CG4411 -2.3 FBgn0039633 CG11873 BKN26700 2.2  
    FBgn0028991 CG4055 HFA06863 -3.7 *
    FBgn0043884 mask HFA16018 -5.6 *
    FBgn0043884 mask HFA16005 -5.8 *
    FBgn0043884 mask BKN20625 -4.3 *
    FBgn0004656 fs(1)h HFA18778 -4.1 *
    FBgn0086902 kis BKN20986 2.3  
    FBgn0004864 hop HFA20340 -5.8 *
    FBgn0004864 hop BKN24272 -5.7 *
HFA12365 Rm62 3.1 FBgn0035720 CG10077 HFA09691 3.1 *
HFA07091 CG8179 4.9 FBgn0020306 dom BKN21379 -3.4  
    FBgn0015618 Cdk8 HFA11113 -2.3  
    FBgn0015903 apt HFA04671 3.2 *
    FBgn0026575 CG4411 HFA19901 -2.1  
    FBgn0260794 ctrip BKN20611 -2.6  
    FBgn0028991 CG4055 HFA06863 -3.7  
    FBgn0039633 CG11873 BKN26700 2.2 *
    FBgn0000008 CG14169 HFA10170 -2.2  
    FBgn0003013 osa HFA17022 -4.2  
    FBgn0051716 Cnot4 BKN22063 2.1 *
    FBgn0260724 larp HFA16984 -2.9  
    FBgn0035720 CG10077 HFA09691 3.1 *
    FBgn0011666 msi HFA17003 -2.6  
    FBgn0003507 srp HFA17068 13.6 *
    FBgn0003507 srp BKN45799 8.6 *
    FBgn0004656 fs(1)h HFA18778 -4.1  
    FBgn0003687 Tbp BKN20836 -4.5  
    FBgn0012049 msi HFA17003 -2.6  
    FBgn0004606 zfh1 BKN29931 5.2 *
    FBgn0001078 ftz-f1 BKN28995 2.3 *
    FBgn0086902 kis BKN20986 2.3 *
    FBgn0034258 CG4954 HFA06905 -5.5  
    FBgn0027492 CkIIbeta HFA20230 -2.2  
    FBgn0032633 CG4055 HFA06863 -3.7  
    FBgn0043884 mask HFA16018 -5.6  
    FBgn0043884 CG6268 HFA16005 -5.8  
    FBgn0043884 mask BKN20625 -4.3  
HFA06863 CG4055 -4.2 FBgn0015618 Cdk8 HFA11113 -2.3 *
    FBgn0000008 CG14169 HFA10170 -2.2 *
    FBgn0003507 srp HFA17068 13.6  
    FBgn0003507 srp BKN45799 8.6  
    FBgn0039633 CG11873 BKN26700 2.2  
    FBgn0001078 ftz-f1 BKN28995 2.3  
    FBgn0086902 kis BKN20986 2.3  
HFA04671 apt 2.7 FBgn0001078 ftz-f1 BKN28995 2.3 *
    FBgn0030636 cngl HFA19089 -2.7  
    FBgn0003507 srp HFA17068 13.6 *
    FBgn0003507 srp BKN45799 8.6 *
HFA18711 RpS14b -6.0 FBgn0004403 RpS14a HFA18710 -4.9 *
HFA17068 srp 8.5 FBgn0030636 cngl HFA19089 -2.7  
    FBgn0020306 dom BKN21379 -3.4  
    FBgn0015618 Cdk8 HFA11113 -2.3  
    FBgn0039633 CG11873 BKN26700 2.2 *
    FBgn0260794 ctrip BKN20611 -2.6  
    FBgn0003016 kis BKN20986 2.3 *
    FBgn0086758 CG17156 HFA00485 7.0 *
    FBgn0086758 CG17649 HFA00509 7.0 *
    FBgn0086758 chinmo BKN45751 6.6 *
    FBgn0005630 lola BKN30256 3.1 *
    FBgn0000259 CkIIbeta HFA20230 -2.2  
    FBgn0028371 CG13525 HFA04167 -2.9  
    FBgn0032633 CG4055 HFA06863 -3.7  
    FBgn0015903 apt HFA04671 3.2 *
    FBgn0026575 CG4411 HFA19901 -2.1  
    FBgn0001078 ftz-f1 BKN28995 2.3 *
    FBgn0011666 msi HFA17003 -2.6  
    FBgn0028991 CG4055 HFA06863 -3.7  
    FBgn0035720 CG10077 HFA09691 3.1 *
    FBgn0000008 CG14169 HFA10170 -2.2  
    FBgn0086902 kis BKN20986 2.3 *
HFA09691 CG10077 2.6 FBgn0003261 Rm62 HFA12365 3.8 *
BKN41059 CG40121 -3.6 FBgn0043903 dome BKN25660 -7.9 *
    FBgn0000259 CkIIbeta HFA20230 -2.2 *
    FBgn0004656 fs(1)h HFA18778 -4.8 *
    FBgn0043903 dome HFA19583 -13.5 *
    FBgn0010412 RpS19 HFA20281 -7.0 *
  1. Bold and * = labelling of off-targets indicates that their knockdown resulted in a significant Z-score in the same direction as the original intended gene.
  2. On-target dsRNAs with potentially interacting off-targets identified in each screen.