Combining laser microdissection and RNA-seq to chart the transcriptional landscape of fungal development

BMC Genomics

Table 1 Summary of sequence reads generated in this study

condition	sample	read length in bases	no. of reads	no. of reads mappedto reference genome	% of reads mappedto reference genome
vegetative mycelium¹	SM1	40	19,709,656	18,881,235	95.7
vegetative mycelium¹	SM6	101	76,664,943	66,365,082	86.5
sexual mycelium²	SM2	35	9,445,238	9,003,807	95.3
sexual mycelium²	SM7	101	68,217,654	60,021,065	87.9
wild-type protoperithecia³	SM4	101	31,837,927	24,722,821	77.6
wild-type protoperithecia³	SM5	101	34,225,766	27,430,438	80.1
pro1 protoperithecia³	SM8	100	52,257,489	30,321,586	58.0
	SM9	100	53,996,808	25,265,576	46.8

The total number of reads is the number of reads after cleaning. Each sample represents an independent biological replicate.
¹Equal amounts of RNA from the following growth conditions (independent biological replicates for each growth condition) were pooled: growth on cornmeal medium for 3d, 4d and 5d, growth on defined medium for 3d, 4d, and 5d; all in shaken cultures.
²Equal amounts of RNA from the following growth conditions (independent biological replicates for each growth condition) were pooled: growth on cornmeal medium for 3d, 4d and 5d, growth on defined medium for 3d, 4d, and 5d; all in surface cultures.
³Laser microdissected samples.

ISSN: 1471-2164