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Table 1 Summary of sequence reads generated in this study

From: Combining laser microdissection and RNA-seq to chart the transcriptional landscape of fungal development

condition sample read length in bases no. of reads no. of reads mappedto reference genome % of reads mappedto reference genome
vegetative mycelium1 SM1 40 19,709,656 18,881,235 95.7
SM6 101 76,664,943 66,365,082 86.5
sexual mycelium2 SM2 35 9,445,238 9,003,807 95.3
SM7 101 68,217,654 60,021,065 87.9
wild-type protoperithecia3 SM4 101 31,837,927 24,722,821 77.6
SM5 101 34,225,766 27,430,438 80.1
pro1 protoperithecia3 SM8 100 52,257,489 30,321,586 58.0
  SM9 100 53,996,808 25,265,576 46.8
  1. The total number of reads is the number of reads after cleaning. Each sample represents an independent biological replicate.
  2. 1Equal amounts of RNA from the following growth conditions (independent biological replicates for each growth condition) were pooled: growth on cornmeal medium for 3d, 4d and 5d, growth on defined medium for 3d, 4d, and 5d; all in shaken cultures.
  3. 2Equal amounts of RNA from the following growth conditions (independent biological replicates for each growth condition) were pooled: growth on cornmeal medium for 3d, 4d and 5d, growth on defined medium for 3d, 4d, and 5d; all in surface cultures.
  4. 3Laser microdissected samples.