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Table 1 The evaluation of microsatellite primer pairs (PPs) for different repeat classes in pooled DNA

From: Development of a genome-wide multiple duplex-SSR protocol and its applications for the identification of selfed progeny in switchgrass

Class

Primer pairs

 

Mean of alleles per locus ± standard error

Tested

Scorable

Percentage (%)

Dinucleotide

409

384

93.9

14.3±0.3

Trinucleotide

369

342

92.7

10.5 ±0.2

Tetra-, penta-,hexa-nucleotide

29

20

69.0

8.3±0.7

Compound

108

96

88.9

10.1±0.3

Total

915

842

92.0

_