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Table 2 Parameters of 48 microsatellite markers assembled into 24 duplexes (Sets 1-24) in switchgrass

From: Development of a genome-wide multiple duplex-SSR protocol and its applications for the identification of selfed progeny in switchgrass

Set

SSR IDa

Typeb

Repeat motif

LGc

Positiond

NAe

Lmin-max (bp)f

Dmin (bp)g

PICh

NE-1Pi

Primer (pmol)j

1

PVAAG-3163/4

gSSR

(ACA)29

5b

63.5

19

211-293

16

0.925

0.248

1

PVGA-1143/4

gSSR

(GA)7-(GA)8

5a

29.3

20

156-195

0.926

0.246

1

2

PVCAG-2397/8

gSSR

(CAG)12

3b

36.3

9

161-189

73

0.822

0.484

2

PVCAG-2517/8

gSSR

(GCT)8

9a

6.9

15

213-234

0.879

0.362

2

3

PVCAG-2147/8

gSSR

(CAG)7

6b

150.5

12

285-306

68

0.836

0.454

1

SWW-1394

eSSR

(GGT)n

7a,VIIa

60.4

8

194-217

0.651

0.709

0.5

4

SWW-463

eSSR

(TG)n

9a

58.3

7

253-273

108

0.762

0.58

0.5

PVAAG-3017/8

gSSR

(AAG)11

6b

58.8

8

132-145

0.787

0.539

0.5

5

PVAAG-3091/2

gSSR

(TTC)13

9a

79.5

13

304-346

71

0.867

0.39

1

PVAAG-3245/6

gSSR

(TTC)9

2a

91.4

19

102-233

0.929

0.237

0.25

6

NFSG-112

gSSR

(GA)n

8b,VIIIb

48

4

189-195

22

0.65

0.472

0.5

NFSG-036

gSSR

(GA)n

4a,IVa

0

17

120-167

0.91

0.289

0.5

7

SWW-573

eSSR

(CAG)n

2b

39.5

11

229-247

60

0.767

0.561

1

PVCAG-2473/4

gSSR

(GCA)12

3a

60.8

13

138-169

0.896

0.325

4

8

PVAAG-2895/6

gSSR

(GAA)10

5b

118

23

310-413

82

0.936

0.215

4

PVCAG-2527/8

gSSR

(GCT)9

4b

60.8

12

206-228

0.867

0.392

4

9

NFSG-200

gSSR

(GA)n

9b,IXb

76.4

15

107-146

19

0.884

0.35

1

NFSG-219

gSSR

(GA)n

8b,VIIIb

26.6

19

165-199

0.928

0.243

1

10

SWW-387

eSSR

(CT)n

9b

46.5

5

154-159

44

0.622

0.49

2

PVGA-1663/4

gSSR

(AG)13

9b

34.1

17

203-230

0.89

0.332

2

11

PVAAG-3051/2

gSSR

(GAA)29

7a

31.8

15

185-254

10

0.851

0.418

1

PVCAG-2209/10

gSSR

(GC)8

4a

13.4

9

264-274

0.839

0.452

1

12

PVCAG-2207/8

gSSR

(CTG)5

2b

40.3

7

221-238

29

0.677

0.685

0.125

PVCAG-2289/90

gSSR

(TGC)5

4b

61

16

157-192

0.886

0.343

0.25

13

PVCAG-2187/8

gSSR

(GCA)7

8b

69.4

13

152-179

99

0.859

0.404

1

PVGA-1549/50

gSSR

(GAA)6

1b

83.5

11

278-331

0.85

0.428

1

14

SWW-2662

eSSR

(AGG)n

2b, IIb

73.5

10

178-197

43

0.774

0.555

1

5211_B07

eSSR

(AGC)8

2a

17.2

7

240-253

0.799

0.525

1

15

PVGA-1357/8

gSSR

(AC)7-(GA)22

5a

59.5

22

229-337

3

0.938

0.211

1

PVGA-1301/2

gSSR

(TC)22

9b

50.5

17

163-226

0.914

0.279

1.5

16

PVGA-1243/4

gSSR

(TC)23

5b

69.3

25

280-317

62

0.945

0.191

2

PVCAG-2297/8

gSSR

(CAG)6

3a

66.6

15

177-218

0.866

0.386

0.5

17

PVAAG-3311/2

gSSR

(CTT)28

2a

29.6

12

140-170

66

0.883

0.353

0.5

PVGA-1813/4

gSSR

(GA)7

5a

72.2

18

236-276

0.915

0.277

1

18

NFSG-035

gSSR

(GA)n

3a, IIIa

129.2

13

117-151

52

0.897

0.325

1

SWW-125

gSSR

(GA)n

2b, IIb

45.5

5

203-220

0.629

0.487

1

19

PVCA-893/4

gSSR

(AC)19

3b

65.3

9

297-336

81

0.78

0.546

1

SWW-1615

eSSR

(GGC)n

1a, Ia

109.8

17

185-216

0.873

0.368

1

20

SWW-1622

eSSR

(GCG)n

2b, IIb

56.3

6

233-246

15

0.77

0.576

1

SWW-1889

eSSR

(GCT)n

6b, VIb

72

4

211-218

0.624

0.484

1

21

PVCA-415/6

gSSR

(TG)16

6a

68.1

20

137-172

5

0.93

0.236

1

SWW-1643

eSSR

(GA)n

3b

136

12

177-207

0.881

0.361

2

22

PVCA-979/80

gSSR

(GT)30

8a

50.2

8

283-310

60

0.678

0.679

2

SWW-2376

eSSR

(CTG)n

5b, Vb

111.5

9

204-223

0.655

0.703

2

23

PVCAG-2269/70

gSSR

(CAG)8

4b

0

17

209-262

6

0.906

0.298

0.5

PVCAG-2361/2

gSSR

(AGC)8

1b

25.9

8

268-277

0.705

0.648

1

24

PVCAG-2279/80

gSSR

(GCT)8

5a

11.7

11

236-248

14

0.83

0.46

0.25

 

PVGA-1963/4

gSSR

(GA)9-(AG)6

5b

66.5

17

191-222

 

0.908

0.294

2

  1. Footnote: a The sequences of primer pairs are available in previous studies [15–19]; b gSSR, genomic SSR; eSSR, EST-SSR; c The linkage group (LG) with an Arabic number indicates the map developed by Liu et al.[24], while Roman number by Okada et al.[18]; d The position indicated genetic distance of linkage map by Liu et al.[24]; e Number of alleles (NA); f Minimum and maximum allele lengths (L); g Minimum difference (D) between non-allelic bands; h Polymorphic information content (PIC); i Average non-exclusion probability for one known parent (NE-1P); j Each primer quantity.
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BMC Genomics

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