Figure 3
From: SERE: Single-parameter quality control and sample comparison for RNA-Seq
Impact of unequal sample sizes. Pairwise comparisons of perfect in silico replicate RNA-Seq datasets were made similarly to Figure2, but keeping the total read count for each pair constant while systematically varying the relative size of both datasets, e.g., 1x106 plus 9x106, 3 x106 plus 7 x106, 5 x106 plus 5 x106. Pearson’s r and Kappa fell for unequal sample sizes demonstrating a maximum for equal sample sizes. SERE remained stable at 1.0 while the 99% CI of repeat measures was optimal (smallest) for equal sample sizes.