Table 2 Quantitative PCR specifics
Putative Gene Annotation | Microarray identifier | EST Accession No. | Sequence (5′-3′)# | Amplicon length (bp) | Amplification Efficiency (%) |
|---|---|---|---|---|---|
ZPC1 | TTC00305 | EC091965 | F – CATACCACCCTTCACCCATC | 212 | 102 |
R – GCTCCACACTAGCCCATGAT | |||||
Vitelline egg envelope gamma | TTC00056 | EC091692 | F – GCTTGCATGTGTCAGGCTTA | 198 | 94 |
R – GGAGAATGGCTTGACTGCTC | |||||
Aveolin | TTC00935 | EC092671 | F – GCTTCTGCTGCTCTTCGTCT | 198 | 90 |
R – AACAACACCTGAGGCAGGAC | |||||
Choriogenin L | TTC04136 | EG630294 | F – GAGCAGTCAAGCCATTCTCC | 230 | 94 |
R – CGTCAATCTCAGTGGCTGAA | |||||
Cathepsin S | TTC00230 | EC091881 | F – GGATCGACACTGGGAACTGT | 297 | 92 |
R – CCCTCCAGTCCAGTGATTGT | |||||
Aquaporin 1 | TTC00180 | EC091829 | F – CCTGTTTCGCAGTCTTGGAT | 191 | 96 |
R – GGTCGGGGTAGGAATCATTT | |||||
Tmc6-related protein 1 | TTC04625 | EG630885 | F – CCGGTTTCTCCTCACCAATA | 135 | 91 |
R – TTGTGCGTGACATTCCTGAT | |||||
Fatty acid-binding protein, adipocyte | TTC00964 | EC092703 | F – ACTGCAATGACCGAAAGACC | 175 | 97 |
R – CCTCCTTTCCGTAGGTCCTC | |||||
Epididymal secretory protein E1 precursor | TTC00209 | EC091860 | F – GCTTGATGGGATTCACCTGT | 215 | 94 |
R – CGATTATTCCCATGGACCAC | |||||
Synaptonemal complex protein 3 | TTC02745 | EC918114 | F – AAGAGCTGAGCGGTTCAGAG | 264 | 91 |
R – TGACCGTGGTAGTTGTTCCA | |||||
T-complex-associated testis-expressed protein 1 | TTC02749 | EC918118 | F – TGCTGGTGAAACACCTCTTG | 208 | 92 |
R – AGGGACAAAAGGGTGGAGTT | |||||
Brain-type fatty acid binding protein | TTC05153 | EG999669 | F – CCTACACCTGATGACCGACA | 212 | 98 |
R – GCTGGGATGATTTGCTCATT | |||||
Intestinal fatty acid-binding protein | TTC05128 | EG999641 | F – CGCAGCGAGAATTATGACAA | 244 | 95 |
R – AGCATGTCACCCTCCATCTC |