Experimental validation of characteristic expression of SPINT2, DUSP6 and PPP1R14A in primary human T helper 2 cells. (A) A histogram overlay showing SPINT2 expression at protein level on the cell surface of the Th cells measured with flow cytometer at 24 hours after activation. (B) SPINT2 secretion from different T helper cells measured with ELISA at 48 hours after activation. * P<0.05, ** P<0.01. A western-blot images showing (C) DUSP6 and (D) PPP1R14A expression on Th cells at 72 h post activation. Representatives of three biological replicates are presented. A house keeping protein GAPDH is shown as a loading control.