Figure 4
Verification of differential methylation of four arbitrarily chosen genes. a. Methylation differences between Red Junglefowl and White Leghorn in three different tissues, estimated by methylation sensitive high resolution melting (MS-HRM) analysis. The table shows the normalized fluorescent intensities together with the statistical analysis of 6-8 individual samples per population, at a temperature where positive and negative methylation controls showed highest intensity difference (Tmd). The right column shows the number of SNPs present in the PCR-product amplified by the primers for the region analysed. b-d, The average methylation level of the promoter regions of three of the genes, FUCA1 (b), PCDHAC1 (c) and RUFY3 (d), in blood samples from F8-generation intercross birds between Red Junglefowl and White Leghorns. The birds differed as indicated in their genotype on an SNP-marker close to the differentially methylated locus.