Table 1 PCR primers for amplification and identification of single nucleotide polymorphisms in UGDH
Amplicon number | Amplicon content | Forward primer sequence | Reverse primer sequence | Amplicon size (bp) |
|---|---|---|---|---|
1 | Exon 1 | GAA TGA ATC CTC CGC CT | TTCCTG TGG TGA TGT TCG | 437 |
2 | Exon 2 | CTTTCCCTATACCTGTCACC | ACTCGTCATCTCACTCCACT | 508 |
3 | Exon 3 | GTTCAGTTGACCTGCTGCTC | CTAGGTGCTCAAGTGTGCTC | 443 |
4 | Exon 4 | GGG CTA CTG ATG ATG TT | CTC TGT CTG GGT TCT TC | 494 |
5 | Exon 5 | AGC AGA AAG TAT TCG TCG | CAG CTT AGC AGC AGG TaG | 450 |
6 | Exon 6 | ACC AAC CCA CCC ATT GCC T | CGG ACA CGA CTG AGC TAC T | 669 |
7 | Exon 7 | AGT TGA TTA GTT TCT TGC | TAA TAA TTG AGT TCC TGG | 744 |
8 | Exon 8 | TTT GGA GAA GTT GGC TAA GG | TGC TAT GCT TCT GAA TGG C | 707 |
9 | Exon 9 | CGT TCA TAT CCT CAC AAG A | TGG GGA AAT CGT TAC AGT A | 522 |
10 | Exon 10 | TTC AAC CAG ACC ATT CCA CT | TGC GTT CAT AAT CCA GTT CC | 339 |
11 | Exon 11 | TAA GGT GAA GTG ACG GAA GC | GGA GCA ACT AAG CCT GTG TG | 473 |
12 | Exon 12 | GGG AATGTA GAC TGA CTC | GCT ACT CAT CCA ATC ACT | 1801 |