Table 2 Information for 10 SNPs in UGDH and allele frequencies in Chinese Holstein population
SNP number | SNP name | SNP location | Position (BTA 6) | Alleles | Frequency | Primer and probe sequence |
|---|---|---|---|---|---|---|
1 | SNP Ex12-3 | Exon 12 | 60966191 | A/T | 0.40/0.60 | F: ATTTCTCGTCCCCCTGAATTGAG |
R: ACGTGGCAGCATAGACAGAAAA | ||||||
P: AAGGCCACACACTGT-MGB | ||||||
2 | SNP Ex12-2 | Exon 12 | 60966411 | C/G | 0.42/0.58 | F: GTACTCTTGCATGGGAAGTTCTACA |
R: CGCTAAGTTGTGTCTGACTCTGTTA | ||||||
P: ACTGTAGCCCGCCAAG-MGB | ||||||
3 | SNP Int11-1 | Intron 11 | 60969505 | A/C | 0.39/0.61 | CAAACAAATG [A/C] AACAAAACCA |
4 | SNP Ex11-1 | Exon 11 | 60969627 | C/T | 0.66/0.34 | F: CCTGTATATGCTCTAATTATTTCTCTGGTTCTT |
R: CGTCAAAGATAAAGGCTGGTTTCAG | ||||||
P: CATTTTTTTATGAATACGTTCAT-MGB | ||||||
5 | SNP Int9-3 | Intron 9 | 60969880 | C/T | 0.59/0.41 | AAATCACTCC [C/T] CTCTTACTCC |
6 | SNP Int9-2 | Intron 9 | 60969929 | G/A | 0.46/0.54 | TCAGACTCTC [G/A] ATTCCTCCTG |
7 | SNP Int5-1 | intron5 | 60975997 | T/C | 0.91/0.09 | GAGAAATAAG [T/C] CCTTGGCTTA |
8 | SNP Inti3-1 | Intron 3 | 60979545 | T/C | 0.32/0.68 | AGCCATAGCA [T/G] TACTTAGTAT |
9 | SNP Ex1-2 | Exon 1 | 61000215 | A/C | 0.75/0.25 | TGGTTGCAGC [A/C] CTCGGTGCCT |
10 | SNP Ex1-1 | Exon 1 | 61000233 | G/A | 0.09/0.91 | GAGCCGAGGA [G/A] ATAGAAGTGG |