Ac/Ds-transposon activation tagging in poplar: a powerful tool for gene discovery

BMC Genomics

Table 2 Copy number of ATDs-rolC in the double transgenic lines, and genomic insertion locus (scaffold and position) with score, e-value and, if applicable, annotated transcript.

Transgenic line	Copy number	Genomic insertion locus (scaffold:position)*	Score	E-value	Transcript (POPTR_)
N82-2	1	18:5,952,389	841.7	0	---
N82-3	1	7:3,347,471	854.3	0	0007s05190 no functional annotation
N82-4	1	8:2,414,980	580.2	5.0e-164	0008s04320 Cyclin, N-terminal domain
N82-5	1	607:11,508	132.9	1.4e-29	0607s00230 no functional annotation
		18:4,994,998	165.4	1.7e-39	0018s05440 Ankyrin Repeat-Containing
N82-7	4	7:9,643,105	71.6	2.5e-11	---
		2:14,852,606	41.0	2.2e-2	---
		n.d.**
N82-8	1	18:1,568,406	1463.8	0	POPTR_0018s01710
		or			F-Box/Leucine Rich Repeat Protein
		3:13,577,759	1551.2	0	POPTR_0003s13180 Heat Shock Protein 90
N82-10	2	2:10,740,018	612.2	5.3e-174	0002s14460 Zinc-finger double stranded
		5:9,290,567	91.5	3.4e-17	0005s12520 GRAS family transcription factor
N82-11	1	16:13,606,858	879.5	0	POPTR_0016s14360 Elongation factor P, C-terminal
N82-12	2	17:7,632,882	44.6	9.8e-3	---
		n.d.**			---
N82-14	2	3:13,408,914	322.3	1.9e-89	---
		n.d.**			---
N82-15	1	2:21,183,733	199.7	6.8e-50	---
N92-1	1	15:6,453,477	286.2	1.6e-75	---
N92-2	1	2:10,740,357	706.4	0	---
N92-3	1	11:16,469,472	39.2	2.0e-3	POPTR_0011s13850 (COBRA-like protein)
N92-4	1	10:15,737,708	515.2	2.1e-144	---
N92-5	1	10:19,481,904	417.9	4.4e-115	---
N92-6	n.d.**	n.d.**
N95-1	1	n.d.**
N95-2	2	25:155,725	1490.9	0	POPTR_0025s00400 (CYTH domain)
		15:824,499	48.2	4.2e-4	---
N95-3	1	9:9,143,423	291.6	3.0e-77	POPTR_0009s10720 (Arylacetamide deacetylase)
N95-4	1	17:7,632,882	44.6	3.2e-3	---
N95-5	1	16:7,726,062	562.1	1.4e-158	---
N95-6	n.d.**	n.d.**

In bold: blast-results with high e-values. In BLAST-analyses where more than one hit was given, either the one with lower e-value or when similar, both hits are shown.
*based on BLAST-results against the genome sequence of P. trichocarpa v2.0 (Phytozome v7.0; http://www.phytozome.net/poplar). Successful positioning of blasted sequence on the physical map of P. trichocarpa was assigned to the Populus-aspen genome because of the high collinearity between the P. trichocarpa and P. tremula/P.tremuloides genomes [49].
**n.d. = not determined.

ISSN: 1471-2164