Strain, cell line, or plasmid | Relevant characteristicsa | Source or reference |
---|---|---|
Escherichia coli | Â | Â |
DH5α | F-, ɸ 80 lacZ DM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdR17 (rK- mK+) supE44 thi-1 ΔgyrA96 relA1 | Laboratory stock |
SY327 | araD Δ(lac pro) argE(Am) recA56 rifr nalA, λ pir | [75] |
Burkholderia cenocepacia | Â | Â |
J2315 | Epidemic strain ET12 clone, CF clinical isolate | P.A. Sokol |
K56-2 | Epidemic strain ET12 clone, CF clinical isolate | BCRRCb |
MH1K | K56-2 ΔBCAL1674-6 | [30] |
JST19 | MH1K ΔBCAL0124 | This study |
JST71 | MH1K ΔBCAS0186 | This study |
JST75 | MH1K ΔBCAM2837 | This study |
JST128 | MH1K ΔBCAL1726 | This study |
JST130 | MH1K ΔBCAM0276 | This study |
JST132 | MH1K ΔBCAM0411 | This study |
JST134 | MH1K ΔBCAM2446 | This study |
JST136 | MH1K ΔBCAM0434-5 | This study |
JST190 | MH1K ΔBCAL0340 | This study |
JST194 | MH1K ΔBCAM1679 | This study |
Macrophage cell lines | Â | Â |
ANA-1 | C57BL/6 murine bone marrow-derived macrophages | [76] |
RAW 264.7 | BALB/c murine macrophage cell line | ATCCc |
Plasmid | Â | Â |
pDA12 | ori pBBR1 , TetR, mob+, P dhfr | [28] |
pDA42 | pDA12, eGFP | [28] |
pDAI-SceI-SacBN | ori pBBR1 , TetR, mob+, P dhfr , sacB, encodes I-SceI endonuclease | [30] |
pDelbcsM | pGPI-SceI with regions flanking BCAL0340 | [77] |
pGPI-SceI | ori R6K , TpR, mob+, carries I-SceI cut site | [74] |
pJT25 | pUC19 with K56-2 rDNA (23S, 16S, 5S), ApR | This study |
pJT31 | pGPI-SceI with regions flanking BCAL0124 | This study |
pJT41 | pGPI-SceI with regions flanking BCAS0186 | This study |
pJT42 | pGPI-SceI with regions flanking BCAM2837 | This study |
pJT45 | pGPI-SceI with regions flanking BCAL1726 | This study |
pJT46 | pGPI-SceI with regions flanking BCAM0276 | This study |
pJT47 | pGPI-SceI with regions flanking BCAM0411 | This study |
pJT48 | pGPI-SceI with regions flanking BCAM2446 | This study |
pJT49 | pGPI-SceI with regions flanking BCAM0434-5 | This study |
pJT52 | pDA12, BCAL1726 | This study |
pJT54 | pDA12, BCAM0411 | This study |
pJT57 | pDA12, BCAL0124 | This study |
pJT71 | pDA12, BCAL0340 | This study |
pJT72 | pGPI-SceI with regions flanking BCAM1679 | This study |
pRK201 | RK2 derivative, KmR, mob+, tra+, oricolE1 | [68] |
pUC19 | ori pMB1 , ApR, mob+, P lac | [78] |