Table 5 Primers for Eimeria tenella protease genes for the amplified coding sequences
From: Stage-specific expression of protease genes in the apicomplexan parasite, Eimeria tenella
Protease gene | Primer | Primer sequence 5′ to 3′ |
|---|---|---|
Aspartic Proteases | Â | Â |
Eimepsin 1 | F | ATC ACC ACA CCA CCA TGG G |
R | CAA GAT TCG AGC AGT TCT CAG CA | |
Eimepsin 2 | F | AGC AAA CAG CTG CAG ATG TTC C |
R | CGA AAA TAG GTC TAG GGG CCC | |
Eimepsin 3 | F | AGA AGT CCT TTC CTC CGT CAC G |
R | GGC GAA GTG TAT TGA AAG CTC G | |
Cysteine Proteases | Â | Â |
Cathepsin B | F | TGA CGT GGG AAG CAG AAG TGT C |
R | ACT GTA TGC ACT CGT GGC GAA A | |
Cathepsin L | F | CAA CCA ACA AGG TCA CTC TTA C |
R | CCC TGG AGG GCC CCC GTG CTC G | |
Cathepsin C1 | F | AAC GGA AGT GGA GAA GAC AGC A |
R | CGG GGT CAA TGA AGG AAG TTT G | |
Cathepsin C2 | F | GGC TTT GAA GTT TGG CAG CG |
R | TGC ATC TGA GCA GCA GAA GAG | |
Cathepsin C3 | F | CGC TCA GGA GTA CAA CTA CGT GGG TGG |
R | GCT GCT GCA CAA GCA GCG CTG CTC TGC C | |
Calpain | F | CGA CTG CTC CTT CCT CTC |
R | GCA TCC TTT AGC TGC TGG | |
Ubiquitinyl hydrolase 1 | F | CTG GCT CTT GAA TGT GCT GCA T |
R | CTT CCA TTT CGT GCC ATT CG | |
Ubiquitinyl hydrolase 2 | F | TTG GAC TTC AGC CCG AGG AG |
R | CCG CTC GTC AAA TCC CAA AG | |
Ubiquitinyl hydrolase 3 | F | AGG TGG TGC CCC TCG ACA TA |
R | TTC TGC TGC CGT GCT CTT TG | |
Ubiquitinyl hydrolase 4 | F | GTG AAT GTG GGC AGC ACC AG |
R | GCT TTC CTG CAA GGC GAA GA | |
Ubiquitinyl hydrolase 5 | F | TCT CTT CCA GGG GCA GTA CAG G |
R | GCT GCT CGT AGG CCA TAT TTG AAC | |
Ubiquitinyl hydrolase 6 | F | GGT GGC CAG CAT AGA CGA GAG T |
R | CCG TAG GTT TGC TGC GAT CTC T | |
Ubiquitinyl hydrolase 7 | F | CGA CAG TCC CGT TGT TGC TG |
R | AGC AAG CCG GGG AAA GAG AC | |
Ubiquitinyl hydrolase 8 | F | TCC TGG AGG CCT TGA GCA GT |
R | TGC TGC TCA CTG GGA TGT GG | |
OTU protease | F | ATG GAC GTA GCA ATC CGT TAC |
R | GTT GCT GCA CAT AGT CCC AAG | |
Pyroglutamyl peptidase | F | ATG GGA CAA CCT ACA GGC GAG |
R | GAG GAA GTT ACA AAC GAA GCA GC | |
Metallo Proteases | Â | Â |
Aminopeptidase N 1 | F | CAA GCA GTA CAC TCC AGC AAC TC |
R | GAA GCG TCA GGT GTT CGT ATT CC | |
Aminopeptidase N 2 | F | GAT ACA TCG CAA GGA CTA CAG C |
R | CAT TGT GCG GAA GGA GTC TG | |
ATP-dependant Zn protease 1 | F | CTT CGA CCA GCT GAA GAT CCT G |
R | TGT CTC CGC GCT AAT GCT G | |
ATP-dependant Zn protease 2 | F | CTC AAT GGT GGA TTT CAG CAA G |
R | CAT CAT TCC CTG GCT TGC G | |
ATP-dependant Zn protease 3 | F | GTC AAG AAA GCA GAT TGT CAG G |
R | CTC GAC AGC CAT CTC AAA GTC | |
CAAX prenyl protease | F | GAA TGT CCC AGG AGA GCT ATG C |
R | GTT CAC AGC GCA CAG AAT TGG | |
Insulysin 1 | F | CAC TTC CTG GAG CAC ATG G |
R | GTG CAG CCT GTC GAA GAT G | |
Insulysin 2 | F | CCG CGG TAG CCT CTCA GC |
R | CTC CGC TGT TGC TCA CAT CTT C | |
Insulysin 3 | F | GAT TGA CGC CGA GCA CCA G |
R | GCT AGG CGA GCT GCT TCA TCC | |
Insulysin 4 | F | GTC GTG CAG AGA GGC TGG |
R | GAG GTA GCT GAG TCG GAG GG | |
Insulysin 5 | F | ATG ATA TTT CTG AAG TCT GC |
R | GAG GAT TCA AAA GAT GGT C | |
Leucine aminopeptidase | F | CAA GAT CTA GTT GTT TGA GGA GCC C |
R | GCT GTT CCA CTT CAC TCG TCT TC | |
O-sialoglycoprotease | F | GCA AAT GTG ACG CAG CTG ATT CG |
R | CGA ACT TGA GGG CCA TTT GCT C | |
S2P-like protease | F | GCC CTC GTA TAA CAG CAA C |
R | GAA CGA CTA ACT TCC TCT TGC | |
Serine Proteases | Â | Â |
Trypsin 1 | F | GTA GGC AAC GGA ACT CCA GC |
R | CGA GGA CTA CAA CTG TCT CC | |
Trypsin 2 | F | ATG GAA GCG TCT GGT TGG GAC |
R | GCT TTT GCT GCA TGC ACT C | |
Trypsin 3 | F | GAC AAA CAA GTT CAA CGA GCA CTG |
R | GCT TCC CTC TCC GCA GAA TTG | |
Subtilisin 1 | F | TAA TTA CCT CCA TCC CGA ACT G |
R | CCA GAA TCT TCA GCG CCA TCA C | |
Subtilisin 2 | F | GCA GCA GCA AAT GTT GAA GAC CC |
R | ATA AGT GCT GCT GCC AAC CAC C | |
Subtilisin 3 | F | AGA GCT TTT GTC CGT GGT GGA G |
R | AAA GAC CCC GAA AAC CAA TGC T | |
Subtilisin 4 | F | CCT TTG TGG CGT GTT CGT GAG |
R | CCA GCA GAA GCA GTA CCG TGG CC | |
Subtilisin 5 | F | TTG AAG CCG ACA GGA CGT GG |
R | CCG CGT AGT CAA GAG CAG GGA T | |
Subtilisin 6 | F | AGC GGC TGC GAC TTG AAC C |
R | CCG TAG CCG CCG TAG GAG TT | |
Prolyl endopeptidase | F | ACA GCC AGG CAC ATC AAT GGT |
R | GCC AAA CCC AAG CCC AGA TAG | |
Clp protease | F | GCT GCA CTT CCA GAA GCG G |
R | CCT CCG CAG AGA AGA CTT TGC | |
Rhomboid protease 1 | F | GGT TGT CCG CAC GTT GGC AG |
| Â | R | CGA AGA TAA CAG GCA CGC AAG ATG |