Consensus clustering identifies 3 distinct REST subgroups. Consensus clustering performed 1,000 iterations of delimiting TCGA tumors (X and Y axes) into 2, 3, 4 and 5 subtypes along a spectrum of REST function. (A) Dark red pixels represent a tumor that is consistently placed in the same group throughout the 1,000 iterations, while lighter pink pixels represent inconsistent cluster placement for tumors. (B) Expression of REST signature genes is significantly lower in the mid-range and REM tumor groups. *p<0.05 when compared to near-normal tumors, **p<0.05 compared to mid-range tumors, ***p<0.05 compared to both near-normal and mid-range tumors. Statistical analysis performed using one-way ANOVA Kruskal-Wallice test and Dunn’s multiple comparisons test in Graphpad Prism 5.01.