Summary of bioinformatics and expression analysis of CSEPs. Identification of CSEPs and family assignment. Workflow used to find and annotate CSEPs in the genome of B. graminis f.sp. hordei. After three iterative rounds of BLAST and annotation, genes were clustered into families as described in Methods. Protein analyses. The proteins predicted to be translated from the CSEP ORFs were analyzed to infer their 3D structure and presence of conserved motifs. These were then used to investigate evidence of different selection pressures during the course of evolution of the CSEP families. Expression analysis. Evidence to support the existence of CSEP genes was obtained from ESTs in the public databases, from RNAseq surveys and from the analysis of the proteomes of B. graminis and B. graminis-infected barley tissues by mass-spectrometry.