Figure 1

Workflow of evaluation of the potential functionomes. Detailed workflow of the three annotation servers, KAAS, MEGAN4, and MG-RAST using query sequences after gene finding process of sequenced data; KAAS and MEGAN4 use BLASTP and BLASTX for amino acid and nucleotide query sequences respectively and the MG-RAST uses only BLASTX. All use different databases, i.e. KEGG GENES for KAAS, NCBI-NR for MEGAN4, and M5nr [15] for MG-RAST (M5nr includes the SEED as a subset.), and different default threshold values for the BLAST hits. Each server converts the hit entries to the corresponding orthology IDs for functional annotation and pathway/module/subsystem mapping. Red colored texts of KAAS indicate its improvements in the current study (see Assignment of the query sequences to KO identifiers in the Methods section).