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Table 2 Strains and plasmids constructed for IRES analysis

From: Comprehensive structural annotation of Pichia pastoris transcriptome and the response to various carbon sources using deep paired-end RNA sequencing

Strains

Plasmids

Short descriptions

X33/pRml-LacZ

pRml-LacZ

Negative control

X33/pRml-UTR-LacZ

pRml-UTR-LacZ

UTR was amplified from KOG1 or GCN2 gene

X33/pLacZ

pLacZ

Positive control of the expression of LacZ