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Table 2 QTL effects validated in backcross populations

From: High-throughput SNP genotyping in Cucurbita pepo for map construction and quantitative trait loci mapping

A Quantitative traits

QTL

Marker1

BCZ2

BCS3

  

a

h

b

h

Flowering

NoMaF_3

C001057

1.273

1.143

3.467

1.929

NoFeF_3

C001057

11.546

9.714

19.400

14.286

DMaF_3

C001057

24.818

23.571

30.200

26.786

DFeF_3

C001057

31.364

27.571

41.000

36.357

N°MaF_3

C001057

19.000

21.000

35.300

30.800

MaF/FeF_3

C001057

3.974

2.531

18.900

11.600

Immature fruits

IFLe_6

C002852

19.633

15.450

7.890

11.556

IFLe_18

C003897

17.717

17.367

8.300

10.500

Mature fruits

MFLe_6

C002852

31.806

26.994

9.728

13.386

MFWi_6

C002852

9.361

10.950

13.469

13.250

MRib_11

C004998

0.000

0.000

2.118

1.500

MCaTh_6

C008686

61.847

75.932

80.080

85.575

MFFi_2

C011474

10.614

10.830

11.648

8.671

MLoN°_5

C016718

3.450

3.500

4.600

3.900

MLRCo_14

C005730

50.285

77.989

82.382

78.662

MaRCo_14

C005730

-7.902

0.419

-0.4406

0.9755

MbFCo_16

C030754

22.338

26.532

10.636

12.139

B Qualitative traits4

     

QTL

Marker1

BCZ2

BCS3

  

a

H

b

h

Vine traits

     

SC_14

C005730

    

dark green

 

0.64

0.29

0.18

0.67

intermediate

 

0.36

0.71

0.64

0.33

light green

 

0.00

0.00

0.18

0.00

P5

 

0.192

0.011†

LIns_14

C005730

    

absent

 

1.00

0.29

0.00

0.00

present

 

0.00

0.71

1.00

1.00

P

 

0.020†

1.00

T_1

C003546

    

present

 

0.58

0.33

0.53

1.00

absent

 

0.42

0.67

0.47

0.00

P

 

0.864

0.011†

Immature fruits

     

IFCo_20

C005014

    

green

 

0.13

0.00

0.00

0.00

light green

 

0.00

0.50

0.00

0.08

white-green

 

0.88

0.50

0.17

0.46

white

 

0.00

0.00

0.83

0.46

P

 

0.051†

0.404

Mature fruits

     

MFSh_6

C002852

    

elongated

 

1.00

0.89

0.00

0.00

pear-shaped

 

0.00

0.11

0.06

0.36

discoid

 

0.00

0.00

0.94

0.64

P

 

0.500

0.042†

MPriRCo_14

C005730

    

black

 

0.91

0.00

0.00

0.00

green

 

0.09

0.00

0.00

0.00

cream

 

0.00

0.29

0.00

0.06

white

 

0.00

0.71

1.00

0.94

P

 

0.000†

1.000

MPSecRCo_14

C004187

    

dotted speckled

 

0.92

0.50

0.17

0.00

banded

 

0.00

0.00

0.00

0.00

striped

 

0.00

0.00

0.00

0.00

absent

 

0.08

0.50

0.83

1.00

P

 

0.022†

0.163

MFCo_16

C017913

    

green

 

0.09

0.00

0.00

0.00

orange

 

0.45

0.00

0.00

0.00

yellow

 

0.27

0.67

0.00

0.00

cream

 

0.09

0.00

0.15

0.00

white

 

0.09

0.33

0.85

1.00

P

 

0.051†

0.222

  1. A. Average data for quantitative traits with QTL displaying significant differences (P > 0.05) between homozygous and heterozygous individuals for linked markers in BC populations. Major QTL (R2> 25%) are indicated in bold. Data traits with significant differences are indicated in bold. B. Frequency for the different categories for qualitative traits with QTL displaying significant differences between homozygous and heterozygous individuals for linked markers in BC populations and results of the Fisher exact test. Data traits with significant differences are indicated in bold.
  2. 1 Tested markers located in the QTL region (see Figures 2, 3, 4, 5 and 6).
  3. 2 Homozygotes for the Zucchini allele of the corresponding marker in BCZ are indicated as a (allele from MU-CU-16), while heterozygotes are indicated as h.
  4. 3 Homozygotes for the Scallop allele of the corresponding marker in BCS are indicated as b (allele from UPV-196), while heterozygotes are indicated as h.
  5. 4 Only the categories represented in the BC populations are included.
  6. 5 Fisher's exact probability test. P (α = 0.05). P < 0.05 implies association and linkage with the marker, as H0 of independence is rejected (†).