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Table 3 List of RT-PCR validated sRNA genes in conserved in Burkholderia and unique to Burkholderia pseudomallei.

From: Computational discovery and RT-PCR validation of novel Burkholderia conserved and Burkholderia pseudomallei unique sRNAs

  Name Chr/Strand Start - end/Length GC content Conservation (Infernal search)
Highly conserved in Burkholderia    
  Bp1_Cand449_SIPHT* 1/- 110185 - 110354/170 50.59% Bacteria (detected in Proteobacteria, Bacteroidetes, Firmicutes, etc)
  Bp1_Cand612_SIPHT 1/- 2290411 - 2290508/98 52.04% Burkholderia
  Bp1_Cand684_SIPHT 1/- 2768674 - 2768787/114 64.04% Bacteria (detected in Actinobacteria, Cyanobacteria, Firmicutes, etc)
  Bp1_Cand697_SIPHT 1/- 2887980 - 2888055/76 64.47% Burkholderia
  Bp1_Cand738_SIPHT 1/- 3154052 - 3154260/209 50.72% Burkholderia
  Bp1_Cand871_SIPHT^ 1/+ 4031759 - 4031986/228 59.21% Burkholderia
  Bp2_Cand287_SIPHT 2/- 2326038 - 2326224/187 62.57% Proteobacteria (predominantly in Burkholderiales, detected in Deltaproteobacteria and Gammaproteobacteria)
Unique to B. pseudomallei     
  Bp2_Cand11_sRNAscanner 2/- 892370 - 892562/193 36.27% B. pseudomallei
  Bp2_Cand77_SIPHT 2/+ 575285 - 575425/141 57.45% B. pseudomallei
  1. * Bp1_Cand449_SIPHT and Bp1_Cand846_SIPHT were verified using the same pair of primers with only Bp1_Cand449_SIPHT also confirmed via sequencing.
  2. ^ Bp1_Cand871_SIPHT was not confirmed via sequencing due to ambiguous sequencing results.