Diagram showing binding pattern of primers used to prepare DNA template of data 1 and data 2. (A) Data 1 used five pairs of overlapping primers to prepare five DNA templates that covered the whole dengue genome. (B) Data 2 combined a set of primers from data 1 to prepare two overlapping fragments. Fw1 and Rv3 were used to amplify a template from the first part of the genome. Fw4 and Rv5 were used to prepare a template from last part of the dengue genome.