CpG coverage in different genomic regions detected by the single-enzyme ( MspI ) and double-enzyme RRBS ( MspI plus ApeKI ) methods. (a) Genomic coverage by single-enzyme (top) and double-enzyme (bottom) RRBS in the YH genome with the same size selection (40-220 bp) and the same read lengths (50 bp). The number of individual CpG dinucleotides measurements in each genomic element, including promoters (2 kb upstream to TSS), CGIs and CGI shores (2 kb adjacent to upstream and downstream of CGIs) are displayed by incremental colors. (b) Genomic CpG coverage (CpG was covered at least once, CpG depth ≥ 1×) by single- or double-enzyme RRBS based on in silico analysis or sequencing data in YH and mDC samples. Ranges of size-selections for enzyme digested fragments and read lengths are displayed.