Figure 5

Expression of HDAC4, CDK6, and SMAD1 in native and osteo-differentiated USSC as well as in response to regulating miRNAs. Western-blots were scanned and protein expression levels quantified, normalized against the housekeeping proteins α-tubulin or ß-actin, and compared to expression in native/untransfected cells (percent values shown below the blot scans). (A) HDAC4 expression in native and osteo-differentiated (day 9) USSC 86b, (B) HDAC4 expression in USSC86b in response to transfection of a miR-29b mimic compared to transfection of a negative control small RNA and untransfected (native) cells. (C) CDK6 expression in native USSC 86b and USSC 77, on days 7 and 12 post DAG induction. (D) CDK6 expression in USSC 86b in response to transfection with miR-26a/b, miR-29b and a negative control small RNA as compared to native cells. (E) SMAD1 expression in native and day 9-osteo-differentiated cells USSC 86b, (F) SMAD1 expression in response to transfection of USSC 86b with miR-26a/b as compared to negative control transfected and native cells.