Table 1 Differentially expressed (>2 log2) genes in HepG2 cells after treatment with Padma 28
| Â | Â | Microarray (n=1) | qPCR (n=5) | ||
|---|---|---|---|---|---|
Gene | Gene title | log2 (ratio) | Fold | Fold | P(H1) |
CYP1A1 | cytochrome P450, family 1, subfamily A1 | 8.7 | 408.4 | 123.4 ± 31.1 | 0.001 |
PTGR1 | prostaglandin reductase 1 | 4.8 | 27.4 | Â | Â |
SLC7A11 | solute carrier family 7, member 11 | 4.2 | 18.9 | Â | Â |
AKR1B10 | aldo-keto reductase family 1, member B10 | 3.9 | 14.7 | 13.9 ± 4.9 | 0.003 |
ASNS | asparagine synthetase | 3.4 | 10.2 | 18.8 ± 5.5 | 0.008 |
CYP24A1 | cytochrome P450, family 24, subfamily A 1 | 3.1 | 8.4 | 5.0 ± 0.9 | 0.132 |
PMAIP1 | phorbol-12-myristate-13-acetate-induced protein 1 | 3.0 | 8.2 | Â | Â |
NUPR1 | nuclear protein 1 | 2.6 | 6.3 | Â | Â |
SCHIP1 | schwannomin interacting protein 1 | 2.6 | 6.2 | Â | Â |
GSTA1* | glutathione S-transferase alpha 1 | 2.6 | 6.1 | 4.5 ± 1.3 | 0.036 |
FECH | ferrochelatase | 2.4 | 5.2 | 3.7 ± 0.8 | 0.018 |
CD14 | CD14 molecule | 2.4 | 5.2 | Â | Â |
AKR1C2** | aldo-keto reductase family 1, member C2 | 2.4 | 5.1 | 6.7 ± 1.2 | 0.007 |
LOC374443 | CLR pseudogene | 2.3 | 5.0 | Â | Â |
BCAT1 | branched chain aminotransferase 1, cytosolic | 2.3 | 5.0 | Â | Â |
KLHDC9 | kelch domain containing 9 | 2.2 | 4.6 | Â | Â |
HELLS | helicase, lymphoid-specific | 2.2 | 4.4 | Â | Â |
CARS | cysteinyl-tRNA synthetase | 2.1 | 4.3 | 3.2 ± 0.6 | 0.001 |
AKR1C1** | aldo-keto reductase family 1, member C1 | 2.1 | 4.2 | 6.7 ± 1.2 | 0.007 |
ABCC4 | ATP-binding cassette, sub-family C, member 4 | 2.1 | 4.2 | Â | Â |
PIR | pirin (iron-binding nuclear protein) | 2.1 | 4.2 | Â | Â |
RAB27A | RAB27A, member RAS oncogene family | 2.1 | 4.2 | Â | Â |
HMOX1 | haeme oxygenase (decycling) 1 | 2.1 | 4.2 | 5.3 ± 0.8 | 0.008 |
GCLM | glutamate-cysteine ligase, modifier subunit | 2.1 | 4.1 | Â | Â |
RBM39 | RNA binding motif protein 39 | -2.8 | -7.2 | 1.1 ± 0.6 | 0.083 |
DKK1 | dickkopf homolog 1 (Xenopus laevis) | -2.7 | -6.7 | -24.2 ± 5.9 | 0.005 |
SCN1A | sodium channel, voltage-gated, type I, alpha subunit | -2.6 | -6.0 | -30.3 ± 10 | 0.002 |
SLC13A3 | solute carrier family 13, member A3 | -2.4 | -5.2 | Â | Â |
MT1M | metallothionein 1M | -2.3 | -4.9 | Â | Â |
LGR5 | leucine-rich G protein-coupled receptor 5 | -2.1 | -4.4 | -8.1 ± 1.9 | 0.001 |
SLC26A3 | solute carrier family 26, member 3 | -2.1 | -4.2 | Â | Â |
SH3PXD2A | SH3 and PX domains 2A | -2.0 | -4.1 | Â | Â |
SUCLG1 | succinate-CoA ligase, alpha subunit | -2.0 | -4.1 | 0.3 ± 0.6 | 0.786 |