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Table 1 Differentially expressed (>2 log2) genes in HepG2 cells after treatment with Padma 28

From: Pathway-focused bioassays and transcriptome analysis contribute to a better activity monitoring of complex herbal remedies

   Microarray (n=1) qPCR (n=5)
Gene Gene title log2 (ratio) Fold Fold P(H1)
CYP1A1 cytochrome P450, family 1, subfamily A1 8.7 408.4 123.4 ± 31.1 0.001
PTGR1 prostaglandin reductase 1 4.8 27.4   
SLC7A11 solute carrier family 7, member 11 4.2 18.9   
AKR1B10 aldo-keto reductase family 1, member B10 3.9 14.7 13.9 ± 4.9 0.003
ASNS asparagine synthetase 3.4 10.2 18.8 ± 5.5 0.008
CYP24A1 cytochrome P450, family 24, subfamily A 1 3.1 8.4 5.0 ± 0.9 0.132
PMAIP1 phorbol-12-myristate-13-acetate-induced protein 1 3.0 8.2   
NUPR1 nuclear protein 1 2.6 6.3   
SCHIP1 schwannomin interacting protein 1 2.6 6.2   
GSTA1* glutathione S-transferase alpha 1 2.6 6.1 4.5 ± 1.3 0.036
FECH ferrochelatase 2.4 5.2 3.7 ± 0.8 0.018
CD14 CD14 molecule 2.4 5.2   
AKR1C2** aldo-keto reductase family 1, member C2 2.4 5.1 6.7 ± 1.2 0.007
LOC374443 CLR pseudogene 2.3 5.0   
BCAT1 branched chain aminotransferase 1, cytosolic 2.3 5.0   
KLHDC9 kelch domain containing 9 2.2 4.6   
HELLS helicase, lymphoid-specific 2.2 4.4   
CARS cysteinyl-tRNA synthetase 2.1 4.3 3.2 ± 0.6 0.001
AKR1C1** aldo-keto reductase family 1, member C1 2.1 4.2 6.7 ± 1.2 0.007
ABCC4 ATP-binding cassette, sub-family C, member 4 2.1 4.2   
PIR pirin (iron-binding nuclear protein) 2.1 4.2   
RAB27A RAB27A, member RAS oncogene family 2.1 4.2   
HMOX1 haeme oxygenase (decycling) 1 2.1 4.2 5.3 ± 0.8 0.008
GCLM glutamate-cysteine ligase, modifier subunit 2.1 4.1   
RBM39 RNA binding motif protein 39 -2.8 -7.2 1.1 ± 0.6 0.083
DKK1 dickkopf homolog 1 (Xenopus laevis) -2.7 -6.7 -24.2 ± 5.9 0.005
SCN1A sodium channel, voltage-gated, type I, alpha subunit -2.6 -6.0 -30.3 ± 10 0.002
SLC13A3 solute carrier family 13, member A3 -2.4 -5.2   
MT1M metallothionein 1M -2.3 -4.9   
LGR5 leucine-rich G protein-coupled receptor 5 -2.1 -4.4 -8.1 ± 1.9 0.001
SLC26A3 solute carrier family 26, member 3 -2.1 -4.2   
SH3PXD2A SH3 and PX domains 2A -2.0 -4.1   
SUCLG1 succinate-CoA ligase, alpha subunit -2.0 -4.1 0.3 ± 0.6 0.786
  1. *primers detect GSTA1-A3 and A5; **primers detect AKR1C1-C4a.
  2. The P(H1) value calculated for the qPCR experiments indicates the probability that the difference between sample and control group is significant.