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Table 4 Summary of BLAST searches of Amblyomma americanum against Ixodes scapularis

From: Why so many unknown genes? Partitioning orphans from a representative transcriptome of the lone star tick Amblyomma americanum

  

UniProtKB

A. americanumESTs

A. Datasets for I. scapularis

 

Annotation

No Annotation

Totals

ESTs

Match

2,997

457

3,454 (24.1%)

 

No Match

1,121

9,735

10,856

Predicted Peptides

Match

3,413

179

3,592 (25.1%)

 

No Match

705

10,013

10,718

Contigs

Match

1,724

642

2,365 (16.5%)

 

No Match

2,394

9,550

11,944

Singletons

Match

3,279

845

4124 (28.8%)

 

No Match

839

9,347

10,186

  

I. scapularis ESTs

  

B. Datasets for I. scapularis

 

Totals

  

Predicted Peptides

Match

128,738 (66.2%)

  
 

No Match

70,722

  

Contigs

Match

72,465 (37.3%)

  
 

No Match

121,995

  

Singletons

Match

105,183 (54.1%)

  
 

No Match

89,277

  
  1. (A) Amblyomma americanum EST sequences matching Ixodes scapularis genomic datasets and (B) I. scapularis EST sequences matching other I. scapularis datasets. Ixodes scapularis datasets include ESTs, predicted peptides, singletons, and assembled contigs. (A) Amblyomma americanum results are categorized according to UniProtKB annotation status (annotation, no annotation) and I. scapularis match status (match, no match). The proportion of the A. americanum ESTs with a match is provided in parentheses for each I. scapularis dataset. (B) BLAST searches of I. scapularis ESTs (N = 194,460) against the three other I. scapularis datasets were then conducted to evaluate the quality of these datasets. These are classified according to match status. The proportion of I. scapularis ESTs with a match is provided in parentheses.
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BMC Genomics

ISSN: 1471-2164

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