Figure 4

Whole genome expression after modulation of miR-30 family expression. miR30 family knock-down was obtained by using 2 oligonucleotides, as described in Methods. A. Efficiency of KD in MCF7 cells was checked by TaqMan qRT-PCR for five members of the miR-30 family. B. a potential new target of miR-30a, AVEN, was also checked. C. Strategy for identification of potential miR-30a targets. As opposed to Figure 3A, class comparison analysis was able to show 330 miRNAs differentially expressed between the 3 classes. 236 genes were commonly upregulated after miR-30 family KD and downregulated after miR-30a overexpression. Out of these, 118 genes contained a miR-30a seed sequence. P value under 0.05 (two-tailed student t test) is represented with a (*).