Figure 5

Sliding-window analysis of Fu and Li’s D for each virus. A. Values of D for DWV. B. Values of D for IAPV. Values were calculated with DnaSP [25] using 200-base windows and a step-size of 25 base. Asterisks indicate windows with statistically significant deviations from neutral expectation at P < 0.05. The outgroup for this coalescent-based statistic is VDV-1 for DWV and KBV for IAPV; gaps introduced by alignment to these outgroups are ignored, resulting in a different alignment length and relative coding positions compared with Figure 1.