(A) Multiple sequence alignment of Reeler proteins of several insect species. The ClustalX program was used for alignments. The GenBank accession numbers for the sequences are as follows: N. lugens (NLU024648.1); B. mori reeler1 (HQ325059); B. mori reeler2 (HQ325058); H. cunea (AAD09280); S. c ricini (BAD05929); A. mylitta (ABG72705); M. sexta (AAO21507); L. obliqua (AAV91350), P. h. corporis (EEB13623); T. infestans (ABR27826); A. pisum (XP_001944294); A. gambiae (EAA14972); T. castaneum (XP_966813), and the reeler domain sequence (Pfam domain PF02014). Black and gray shading indicates the identity and high conservation of amino acids, respectively. The predicted signal peptide sequences of the deduced N. lugens Reeler protein is underlined. Dark gray bars under the sequences indicate the reeler domain regions. (B) Schematic representation of the reeler genes of several insect species. The orange boxes indicate the exon sizes and location of each reeler gene on scaffolds. The deduced Reeler proteins are shown in the below panel. Red and blue bars indicate the putative signal peptide sequence and the putative reeler domains. The size bar indicates the nucleotides of insect reeler genes. (C) Phylogenetic analysis of reeler domains of several insect species. The phylogenetic tree was constructed by Maximum likelihood using the program Mega 5.05 (http://www.megasoftware.net/). The Jones-Taylor-Thornton (JTT) for amino acid substitution model was used, a test of phylogeny was done by the bootstrap method with 1000 replications, bootstrap values>50% are shown on each node of the tree.