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Figure 4 | BMC Genomics

Figure 4

From: Efficient recovery of proteins from multiple source samples after trizol® or trizol®LS RNA extraction and long-term storage

Figure 4

Performance of the modified TRIzol protocol evaluated by immunoblotting. To compare the modified TRIzol protocol versus the TRIzol® manufacturer’s, mirVana™ PARIS™ kit and standardized laboratory (A+2X Buffers) protocols, 106 HCT116 cells were plated in 14 independent 100 mm plates (one per experimental setting: lanes 1 to 14), and cells were processed for total protein extraction 48 h after plating. Next, 40 μg of total protein extracts were separated on 8% SDS-PAGE and transferred onto nitrocellulose membrane. Immunoblot analysis of steady-state expression levels of housekeeping proteins (β-actin and GAPDH), NF-κB, IκB, p-Akt, Akt, p53, and nuclear PARP.

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BMC Genomics

ISSN: 1471-2164

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