Figure 6

Steady-state protein expression levels in human colon cancer samples. Protein extracts were obtained from human colon cancer samples stored in TRIzol^®-chloroform samples at −80°C for 2 years. Forty μg of total protein extracts were separated in 8% SDS-PAGE, transferred to nitrocellulose membrane and evaluated for the steady-state protein expression levels. (A) Representative imunoblots of steady-state expression levels of p53, NF-κB, IκB, p-Akt and Akt, in HCT116 colon carcinoma cells, normal colon, colon adenomas and colon pMMR and dMMR carcinomas. (B) p53 protein steady-state levels in normal colon, colon adenomas, and pMMR and dMMR colon carcinomas; (C) NF-κB, IκB, and NF-κB/IκB ratio; and (D) p-Akt, Akt, and p-Akt/Akt ratio. Results are expressed as mean ± SEM for similar samples. *p < 0.01 and †p < 0.05 from normal colon; §p < 0.01 and ‡p < 0.05 from colon adenomas; #p < 0.01 from colon sporadic dMMR carcinomas.