Figure 1

Characterization of the cellular model. (A) RT-qPCR analysis of epidermal basal cells markers (PCNA, KRT14) and suprabasal cells markers (IVL, KRT1) between proliferating and 3-days differentiated keratinocytes (n=3; error bars represent SD; bilateral paired t-test: *p≤0.05, **p≤0.01). (B) Viability assay between 6Gy-irradiated and sham-irradiated proliferating keratinocytes. Results are expressed as the mean percentage of viable cells assuming 100% viability for non-irradiated cells (n=3; error bars represent SD; bilateral paired t-test : *p≤0.05, **p≤0.01, ***p≤0.001). (C) Viability assay between 6Gy-irradiated and sham-irradiated differentiated keratinocytes. Results are expressed as the mean percentage +/− SD of viable cells of 4 independent experiments assuming 100% viability for non-irradiated cells (bilateral paired t-test: *p≤0.05).