The transcript catalogue of the short-lived fish Nothobranchius furzeriprovides insights into age-dependent changes of mRNA levels

Table 1 N. furzeri transcriptome libraries and sequencing methods

Library	Specimens used for RNA-isolation	Sequencing technology	Number of reads	Average length (bp)	Total length (Mb)
1	one male GRZ, 9 weeks, whole body; normalised^a	Sanger	131,808	734	97
2	one male GRZ, 10 weeks, whole body; normalised^a	454/Roche GS-FLX	751,885	223	168
3	one male GRZ, 10 weeks, whole body	454/Roche GS-FLX	415,426	225	94
4	six male and female GRZ, 8 weeks, whole body; normalised^b	454/Roche GS-Titanium	2,351,236	369	869
5	three GRZ, 1 week, whole body	Solexa/Illumina, 2 x 150 bp	89,730,910	151	6,774
6	five male GRZ, 5 weeks, skin	Solexa/Illumina, 2 x 101 bp	55,410,042	101	5,596
		Solexa/Illumina, 76 bp	33,947,044	76	2,579
7	five male GRZ, 5 weeks, brain	Solexa/Illumina, 2 x 101 bp	60,997,820	101	6,161
		Solexa/Illumina, 76 bp	36,117,265	76	2,744
8	three male GRZ, 14 weeks, skin	Solexa/Illumina, 76 bp	35,963,921	76	2,733
9	three male GRZ, 14 weeks, brain	Solexa/Illumina, 76 bp	27,369,310	76	2,080
10	four male MZM-0403, 5 weeks, skin	Solexa/Illumina, 76 bp	31,727,935	76	2,411
11	five male MZM-0403, 5 weeks, brain	Solexa/Illumina, 76 bp	34,720,289	76	2,638
12	five male MZM-0403, 31 weeks, skin	Solexa/Illumina, 76 bp	30,268,649	76	2,300
13	three male MZM-0403, 31 weeks, brain	Solexa/Illumina, 76 bp	31,423,327	76	2,388
			467,676,512		39,632

ISSN: 1471-2164