Deep sequencing and genome-wide analysis reveals the expansion of MicroRNA genes in the gall midge Mayetiola destructor

Table 1 Growth stage variation in miRNA abundance

miRNA	Hybridization-signal intensity (average ± standard deviation)		Fold change
	1-day larvae	3-day larvae
Abundance decreases with time
mde-miR-87a-3p	2485 ± 389	1706 ± 266	−1.46 ± 0.13
mde-miR-375-3p	4461 ± 466	3461 ± 471	−1.29 ± 0.11
mde-miR-10-5p	2808 ± 366	1273 ± 161	−2.21 ± 0.17
mde-miR-11-3p	1135 ± 200	612 ± 100	−1.85 ± 0.08
mde-miR-34-5p	1860 ± 181	1168 ± 131	−1.59 ± 0.13
mde-miR-1000-5p	943 ± 169	388 ± 98	−2.43 ± 0.19
mde-miR-137-3p	2728 ± 331	1518 ± 228	−1.8 ± 0.05
mde-miR-190-5p	2507 ± 400	701 ± 143	−3.58 ± 0.21
mde-miR-970-3P	3764 ± 501	2821 ± 318	−1.33 ± 0.14
dmo-miR-284	1132 ± 108	443 ± 76	−2.56 ± 0.22
mde-miR-316-5p	182 ± 35	12 ± 10	−15.17 ± 3.1
Abundance increases with time
mde-miR-92a-3p	861 ± 100	1294 ± 143	1.5 ± 0.09
mde-miR-276a-3p	2199 ± 279	3536 ± 368	1.61 ± 0.14
mde-miR-305-5p	1305 ± 257	2696 ± 265	2.07 ± 0.17
mde-miR-79-3p	262 ± 51	432 ± 69	1.65 ± 0.12
mde-mir-92b-3p	551 ± 70	928 ± 114	1.68 ± 0.09
mde-miR-9a-5p	952 ± 113	1689 ± 268	1.77 ± 0.13
mde-miR-9c-5p	468 ± 80	1639 ± 208	3.5 ± 0.22
mde-miR-965-3P	190 ± 32	514 ± 58	2.71 ± 0.19
dme-miR-289	5333 ± 403	19781 ± 927	3.71 ± 0.25

RNA samples were extracted from 1-day and 3-day old larvae, converted to cRNAs, and hybridized to microarrays. Data were derived from three biological replicates with three duplicates for each replicate. Rows in boldface represent hybridization results of probes produced according to miRNAs from other insect species, to which the corresponding miRNAs from Hessian fly have not yet been identified. P ≤ 0.05.

ISSN: 1471-2164