Figure 5

Changes in interferon stimulated gene (ISG) expression and cell death signalling in RSV-infected macrophage cells. (A) Activation of the STAT-1 signalling pathway in RSV-infected PMΦ cells. Cells were infected with RSV and at between 0.2 and 24 hrs post-infection hpi the cells were harvested and the relative STAT-1 and PSTAT-1 levels determined by immunoblotting using relevant antibodies. (B) The fold change (FC) in the expression of interferon stimulated genes and cell death signalling related genes at 4 and 24 hpi in virus-infected cells compared with mock-infected cells is represented as a heat map. In each case the FC range, the probe identification (Probe ID) and gene name (Gene Title) are indicated. (C) Activation of the JNK signalling pathway in RSV-infected PMΦ cells. Cells were infected with RSV and at between 0.2 hpi and 24 hpi the cells were harvested and the relative JNK and phosphorylated JNK levels determined by immunoblotting using relevant antibodies. The mock-infected cell lysate (M) was harvested at 12 hpi.