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Table 6 Correlation of expression of 18 randomly-selected genes among 5 ′ -SAGE, microarray and real-time RT-PCR

From: 5'-Serial Analysis of Gene Expression studies reveal a transcriptomic switch during fruiting body development in Coprinopsis cinerea

 

Description

5′-SAGEa

Microarraya

Real-time RT-PCRa

CC1G_04976

Thiazole biosynthetic enzyme

6.48

3.34

3.80

CC1G_03790

Glutamate decarboxylase

5.55

1.64

3.55

CC1G_00577

CMGC/MAPK protein kinase

4.41

1.41

2.76

CC1G_03377

Protoporphyrinogen oxidase

3.41

−0.17

1.66

CC1G_00689

Thioredoxin

3.16

0.72

2.91

CC1G_07379

Hypothetical protein

1.57

0.19

1.59

CC1G_06934

Hypothetical protein

1.06

−0.85

1.34

CC1G_02958

Actin cytoskeleton protein VIP1

0.82

0.79

0.92

CC1G_10110

Ubiquitin-carboxy protein fusion

−0.05

−0.35

−0.32

CC1G_03278

ATP synthase subunit 5

−0.07

−0.21

−0.41

CC1G_07540

60S ribosomal protein L34-b

−0.16

−0.64

−0.45

CC1G_07277

40S ribosomal protein S14

−0.95

−0.41

−0.78

CC1G_01940

ATP-synthase delta-subunit

−1.34

−0.73

−1.23

CC1G_03707

Prohibitin

−1.43

−0.37

−1.44

CC1G_09336

Basic leucine zipper & W2 domain-containing protein 2

−1.97

−1.17

−1.98

CC1G_06761

Glycine dehydrogenase

−4.42

0.45

−0.82

CC1G_14100

Hypothetical protein

−5.82

−1.53

−3.42

CC1G_01315

Endochitinase

−6.63

−1.45

−4.66

 

Correlation of 5′-SAGE and real-time PCR: R=0.936

 

Correlation of 5′-SAGE and microarray: R=0.792

  1. a Values expressed as log2 ratio of Myc/S1-Pri.