Identification of alternative splicing. A. Statistical analysis of alternative splicing identified from RNA-Seq data. Four different types of alternative splicing were identified. Blue bars represent the number of genes that are alternatively spliced. Orange bars represent the total number of alternative splicing events. B. FGSG_05122 is an example of a gene showing intron retention. There are four reads representing the intron splice sites (green letters). Meanwhile, several reads uniquely match to the intronic region. Black letters represent exonic region; orange letters represent intronic region. C. Three genes with intron retention were selected for confirmation by RT-PCR. Primers were designed flanking the intronic regions. Gene FGSG_10264 was included as a control.