Figure 5

Example of alternative 3′ splicing. A. Three different 3′ intron splice sites were identified in the intron of gene FGSG_06760. There are seven reads showing the reference 3′ intron splice site, 16 reads showing alternative 3′ intron splice site 1, and one read showing alternative 3′ intron splice site 2. Black letters represent the exonic region; orange letters represent the intronic region; green letters represent different 3′ intron splice sites. B. RT-PCR confirmed the alternative 3′ splicing events in the intron of FGSG_06760. C. Protein alignment shows that 16 or 17 amino acids located between coiled coil and HMG domain are lacking in the proteins encoded by the two alternatively spliced transcripts.