Comparative genome analysis of Spiroplasma melliferumIPMB4A, a honeybee-associated bacterium

BMC Genomics

Table 1 Genome assembly statistics

	S. melliferumIPMB4A	S. melliferumKC3	S. citriGII3-3X
Number of chromosomal contigs	24	4	39
Combined size of chromosomal contigs (bp)	1,098,846	1,260,174	1,525,756
Estimated chromosomal size (bp)	1,380,000	1,430,000	1,820,000
Estimated coverage (%)	79.6	88.1	83.8
G+C content (%)	27.5	27.0	25.9
Coding density (%)	85.1	83.0	80.2
Protein-coding genes	932	1,222	1,905
Length distribution (Q1/Q2/Q3) (a.a.)	176/280/440	119/233/376	83/149/286
Plectrovirus proteins	11	132	375
Hypothetical proteins	337	485	519
Annotated pseudogenes¹	12	12	401
rRNA operon	1	1	1
tRNA	32	31	32
Number of plasmids	0	4	7

¹For S. melliferum IPMB4A, putative pseudogenes were annotated with the’/pseudo’ tag in gene feature as suggested by the NCBI GenBank guidelines and were not counted in the total number of protein-coding genes. For S. melliferum KC3 and S. citri GII3-3X, putative pseudogenes were annotated by adding the term ‘truncated’ in the CDS product description field and were included in the total number of protein-coding genes.

ISSN: 1471-2164