Strand-specific PCR of 3 tissue-specific mouse probesets. Panel A shows 25 cycles of amplification. Panel B showed 38 cycles of amplification. Probesets ID 4544718, 4628230, and 4772779 showed tissue-specific expressions to mouse embryo, mouse kidney, and mouse heart respectively. Probesets ID 4544718 targets the last exon of myosin regulatory light chain 2, skeletal muscle (Mylpf) gene. Probesets ID 4628230 targets the 4th exon of inositol oxygenase (Miox) gene. Probesets ID 4772779 targets the 2nd exon of creatine kinase M-type (Ckm) gene. Microarray expression data for these 3 probesets are given in Figure 3.